Today we ran another protein PAGE, but in this case it was a denaturing gel as opposed to a native one. We denatured both, our GFP samples and our standards by heating the samples at 95º C for 2-5 min.
We will compare the results of the native and denaturing gels in terms of the estimates that they provide of the size (molecular weight in Daltons)of GFP.
Since we are one lab ahead, and the next couple of labs must be done back to back, we will not be having a lab session tomorrow Friday.
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