Today we started the process to purify the Green Fluorescent Protein (GFP) from the bacteria we transfromed last week.
The process was really short: Just picking a colony from each of the LB/amp/ara and LB/amp plates, usit to inoculate a tube with a few mLs of LB/amp/ara broth, and sticking the tubes in a shaker/incubator at 32ºC and an undisclosed number of RPMs. Undisclosed, because the knob is not in RPMs, but through experience I would estimate that we set it at about 200 rmps.
The tubes were set so they are in a diagonal position, so the surface area of the medium is larger than in vertical position, promoting a better uptake of O2 from the air, thus enhancing the efficiency of bacterial growth.
Tomorrow morning we will do the actual chromatography to isolate the GFP.
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