We have completed a cycle, and after genetically tranforming bacteria using Bio-Rad's pGLO pasmid, it's time to isolate it from our clones. We used the Wizard® Plus Minipreps DNA Purification System (Promega), which provides a simple and reliable method for rapidly isolating plasmid DNA. This system can be used to isolate any plasmid but works most efficiently when the plasmid is <20,000bp. Click here to view a pdf of the complete protocol of the miniprep kit.
We lysed the bacteria and added a series of reagents to isolate plasmid DNA wothout getting any bacterial genomic DNA. The ultimate objective is to isolate the GFP gene from the rest of the plasmid.
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