LAB - Silver staining a polyacrylamide gel

Download the Bio-Rad Silver Stain Plus protocol in pdf format by clicking here.

pGLO plasmid restriction digest, PAGE, and visualization through silver staining.
The staining procedure didn't work as expected (due to longer than needed staining and shorter than required drying...), so no picture can be taken. Here are drawings of the band patterns:

• How many bands did you expect based on the number of restriction sites for BamH I? (check your plasmid map)
• What may explain the band pattern you got?

Speculate about these questions in your discussion.

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