Thursday Sep 10
Using the micropipette
On Thursday students got acquainted with their new best friend, the micropipette. We did a couple of exercises to make sure measurements were made accurately and that students learned how to use a combination of micropipettes to measure different values. The report was handed in at the end of the lab.
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Friday Sep 11
Restriction Enzyme Digestion (RED) and analysis of lambda DNA through gel electrophoresis
In this lab we used three different restriction enzymes EcoRI, PstI, and HindIII to digest (cleave) DNA from the lambda bacteriophage. As a DNA marker, or DNA "ladder", we used a sample of lambda DNA pre-digested with HindIII.
Students will measure the distance bands in the gel migrated and will infer the size of the different bands based on such information.
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2 comments:
A lab pipette is one of the most extensively used tools in a laboratory. This instrument is used to transfer liquids or semi solids in measured volumes from one container to the other. These products have also found extensive use in the field of molecular biology where measured amounts of samples need to be extracted and introduced into lab machines that correspond to their use.
Hello,
Useful information, many thanks to the author. It is puzzling to me now, but in general, the usefulness and significance is overwhelming. Very much thanks again and best of luck!
Micropipette
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