We performed the genetic transformation of E. coli cultures with BioRad's pGLO™ plasmid, engineered to contain the green fluorescent protein (GFP) gene , originally isolated from the crystal jelly Aequorea victoria.
We also performed the ligation of our GAPC gene (obtained in lab 05) with BioRad's pJet1.2 plasmid. In the next lab we will genetically transform bacteria, just as we did earlier today.
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