Lab 12 - pGLO Small Scale Plasmid Purification Lab 13 - pGLO Restriction Enzyme Digestion (RED)

pGLO plasmid and restriction map
___________________________________________

Today we used the bacteria we transformed with the pGLO plasmid and cloned a few weeks ago to perform a small scale plasmid DNA purification (minipreps) and isolate the pGLO plasmid again.

Then we performed a restriction enzyme digestion, RED, using the restriction enzymes EcoRI and HindIII (see restriction map).

----------------------