Saturday, October 10, 2009

Labs 07 and 08

Friday October 09 2009

Lab 08 - Ligation and transformation

Students used bacterial colonies from the LB/Ampicillin agar plates incubated yesterday to inoculate LB/Ampicillin broth media. The broth media will be incubated for approx. 24 hours at 37ºC in a shaking water bath at approx. 200 rpm.


Lab 07 - Protein purification by chromatography

Students used the bacterial cultures inoculated yesterday to isolate Green Fluorescent Protein (GFP) by the process of Hydrophobic Interaction Chromatography (HIC).

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