Thursday, November 5, 2009

Lab 03 - PCR of the PV92 Alu insertion locus (gel electrophoresis)
Lab 14 - Size exclusion chromatography (SEC)

Today we ran a gel to confirm the results of the PCR in which the target was the PV92 Alu insertion locus in our own DNA. Results will be published soon...

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Students also did lab 14, size exclusion chromatography (SEC). It is the second kind of chromatography that we have performed (the first one was hydrophobic interaction chromatography HIC)).
The kit provided a mix of hemoglobin and Vitamin B12, proteins that have very different sizes and can be easily separated using the featured technique.

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