Two-dimensional polyacrylamide gel electrophoresis from E. coli protein extracts
(From Alberts et al. 2008. Molecular Biology of the Cell, 5th edition. © Garland Science)
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Today we started discussing the topic of molecular techniques. There is no single chapter associated in the textbook. The material is a summary taken from miscellaneous sources.
We covered the basics on techniques on protein analysis. We focused on column chromatography (HIC, SEC, Ion-exchange, and affinity), two-dimensional gel electrophoresis and western blotting.
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