Module 2, Lab 06 - Cloning (GAPC gene from Arabidopsis and pJet1.2 plasmid)

Today we used the E. coli cultures you inoculated yesterday to do a small-scale plasmid DNA extraction ('miniprep') using Promega's Wizard® Plus SV Minipreps DNA Purification System.

Here's a recap of what we have done so far in the last couple of exercises:

Lab 5 - Nested PCR of the GAPC gene from Arabidopsis

Lab 6 - Cloning

• Ligation of PCR amplified GAPC gene onto the pJet1.2 plasmid
• Genetic transformation of E. coli with the pJet1.2 plasmid
• Cloning of genetically transformed E. coli
• Minipreps (purification of pJet1.2 plasmid)

Next, a restriction enzyme digestion to confirm successful plasmid DNA purification (lab 6) and setting sequencing reactions (lab 7)

-----------------------