Lecture - Molecular techniques (nucleic acids)

In todays lecture we focused on techniques that are used for analyzing nucleic acids. I decided to focus mainly on DNA sequencing, given how important that such technique has become in the last decade. Topics that were discussed:

• PCR
• PCR in disease diagnosis
• DNA sequencing
• Chain termination method (Sanger method - manual and automated)
• Shotgun sequencing
• Pyrosequencing
• Next-generation sequencing
• DNA typing

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