Today we almost finished lab 08, the ligation and transformation module (ligation of the Arabidopsis GAPC gene into the pJet1.2 plasmid; transformation of E. coli).
We did a plasmid DNA extraction (minipreps) from the bacterial cultures we did last week, and initiated a restriction enzyme digestion with BglII to confirm if we have plasmid DNA and the insert we are interested in. Tomorrow we will run an agarose gel electrophoresis to confirm the results.
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