Friday Oct 16 2009
Today we ran gel electrophoreses to confirm if the plasmid extraction (lab 8) was successful and if we had the GAPC gene (from Arabidopsis) insert. In some cases we did.
Using the samples that had the insert we mixed the miniprep DNA with forward and reverse sequencing primers (pJET SEQ F and pJET SEQ R), and put them in a 96-well plate. The plate will be shipped to the DOE Joint Genome Institute (JGI) to be sequenced as part of their Sequencing Training Program (STR). The results should be in in two weeks, ready to be used in the bioinformatics labs
The report from lab 9 will be merged with the report of lab 15 (Bioinformatics)
While the gels were running we discussed the DNA sequencing technique most commonly used: Dye-terminator sequencing, a modification of Sanger's chain termination sequencing protocol, which allowed the automation of the sequencing process.
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