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Today we finished chapter 5, on DNA replication. We went into details of how the lagging strand is synthesized, forming the Okazaki fragments, thanks to DNA looping, which allows for it's synthesis even though the helicase and polymerase III-dimer complex travels in the same direction of the replication fork. We added how the RNA primers are degraded and the Okazaki fragments are joined, by the action of ribonuclease H, DNA polymerase I, and DNA ligase.
We then discussed how DNA transcription proceeds in eukaryotes, what's the role of telomerase in reparing telomeres after each round of replication, and the differences between the action of polymerases in prokaryotes and eukaryotes.
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