Wednesday, January 6, 2010

Module 2, Lab 06b - Nested PCR of the GAPC gene of Arabidopsis thaliana (second round)

Today, under the direction of Dr. Dennis DeLuca (since I was out of town) students set the second round of PCRs to amplify the GAPC gene of Arabidopsis.

Primers of the first round were inactivated with the use of an exonuclease, and the exonuclease was inactivated through an incubation at 8oºC.

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