Lab 7b - Smal scale plasmid purification (pJet1.2 with GAPC gene insertLab 13 Small scale plasmid purification (pGLO plasmid)

Today we used the bacteria we transformed (and cloned) with the pGLO and pJet1.2 plasmids to perform small scale plasmid DNA purifications (minipreps) and isolate the plasmids.

Then we performed restriction enzyme digestions, RED, using the restriction enzymes Ec0RI and HindIII (see restriction map) for the pGLO plasmid, and BglII for the pJet1.2 plasmid.

The idea was to isolate the GFP gene in a fragment, in the case of pGLO, and to confirm the success of the ligation procedure in the case of pJet1.2.

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