Lab 06 - Bacterial genetic transformation with pGLO Lab 08 - Ligation and transformation (gene GAPC)

We performed the genetic transformation of E. coli cultures with BioRad's pGLO™ plasmid, engineered to contain the green fluorescent protein (GFP) gene , originally isolated from the crystal jelly Aequorea victoria.

We also performed the ligation of our GAPC gene (obtained in lab 05) with BioRad's pJet1.2 plasmid. In the next lab we will genetically transform bacteria, just as we did earlier today.

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Lab 05 - GAPDH Nested PCR

Thursday, October 1st 2009

We ran the gels for the nested PCRs we performed last week.

Due to failure of starter bacterial cultures we couldn't start lab 6, on bacterial genetic transformation, but we did talk about the main topics the lab is related to: Gene regulation, antibiotic resistance, and genetic transformation.

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Exam 1

Today we had our first partial exam.

Results and stats:

(click image for full size view)

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Lecture, Chapter 6 - Transcription

After finishing chapter 5, on the few details that differ in eukaryotic DNA replication compared to that in prokaryotes, we started chapter 6, on transcription.

We talked about the basics of the synthesis of transcripts, and got acquainted with some new terms like cistron, open reading frame (ORF), monocistronic and polycistronic mRNAs, and operon.

Tomorrow: Exam 1...!!!

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