Lecture - Molecular techniques

Today we had a discussion in which we summarized some of the most important techniques that are used to study proteins and nucleic acids. Topics that were covered:

• Protein purification
• Denaturing PolyAcrylamide Gel Electrophoresis (SDS-PAGE)
• Two-dimensional electrophoresis
• PCR
• PCR in disease diagnosis
• DNA sequencing
• Chain termination method (Sanger method - manual and automated)
• Shotgun sequencing
• Pyrosequencing
• Next-generation sequencing
• DNA typing

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6th ONU Intro Molecular Biology SymposiumSecond session

Department of Biological and
Allied Health Sciences
Mathile Center 108
February 14 & 17, 2011

The ONU Intro Molecular Biology Symposium takes place every Fall and Winter quarters, as part of the Introduction to Molecular Biology (Biol 217) class. Speakers are students registered in the class, who throughout the quarter have written a review paper on molecular biology-related topics.

Click on pic for a full size image

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Lecture, chapter 12 - Processing of RNA

Today we talked about how introns are removed by using a combination of small nuclear RNA (snRNA) and proteins called small nuclear ribonucleoproteins (snRNP, a.k.a. "snurps").

Then we discussed alternative RNA splicing mechanisms:

• Alternative promoter selection
• Alternative tail site selection
• Alternative splicing by exon cassette selection
• Trans-splicing (rare)

On Friday: Molecular techniques!

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6th ONU Intro Molecular Biology SymposiumFirst session

Monday, February 14, 2011

Department of Biological and
Allied Health Sciences
Mathile Center 108
February 14 & 17, 2011

The ONU Intro Molecular Biology Symposium takes place every Fall and Winter quarters, as part of the Introduction to Molecular Biology (Biol 217) class. Speakers are students registered in the class, who throughout the quarter have written a review paper on molecular biology-related topics.

Click on pic for a full size image

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