Module 4, Lab 13 - Links for Bioinformatics lab 1

• National Center for Biotechnology Information (NCBI)
• European Bioinformatics Institute (EBI)
• DNA Data Base of Japan (DDBJ)

• Fasta format description
• ClustalW2 (EBI)

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Module 3, Lab 12 - RED of pGLO plasmid

Today we did a restriction enzyme digestion (RED) of the plasmid DNA purifications (minipreps) we did last week. The goal was to isolate the GFP gene from the pGLO plasmid. After the cloning process that would have been a step before purifying the GFP gene sample for further study.

We used the restriction enzymes EcoRI and HindIII (individually and in combination) to reach our goal. We confirmed the results with an agarose gel electrophoresis (students in section 2 even did a "retrophoresis"... Hopefully it was a very valuable lesson)

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Lecture, chapter 11 - Gene regulation at the mRNA level

Today we covered chapter 11, on gene regulation at the mRNA level. Regulation mechanisms in the middle ground between transcriptional regulation and translational regulation.

We discussed the control of rate of degradation of mRNA, the effect of translational regulatory proteins (activators and/or repressors), regulation by anti-sense RNA, and regulation by alterations to the ribosome.

Tomorrow we will discuss RNA interference (RNAi)

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