Today we had our first exam of the quarter.
Statistics:
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Friday, January 8, 2010
Module 2, Lab 06c - Nested PCR of the GAPC gene of Arabidopsis thaliana
Thursday, January 7, 2010
Today we ran a gel electrophoresis with the second round PCRs of the Arabidopsis GAPC gene. Once we confirmed the results of the PCRs we purified the samples to get them ready for ligation and transformation in the next labs.
The starter E. coli agar plates that we were supposed to use to begin lab 9 did not work and will be repeated. On Monday we'll meet to do the genetic transformation of E. coli with the pGLO plasmid, on Tuesday we will inoculate liquid media and on Wednesday we will do lab 10: purification of the green fluorescent protein (GFP) through hydrophobic interaction chromatography.
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Wednesday, January 6, 2010
Module 2, Lab 06b - Nested PCR of the GAPC gene of Arabidopsis thaliana (second round)
Today, under the direction of Dr. Dennis DeLuca (since I was out of town) students set the second round of PCRs to amplify the GAPC gene of Arabidopsis.
Primers of the first round were inactivated with the use of an exonuclease, and the exonuclease was inactivated through an incubation at 8oºC.
Monday, January 4, 2010
Lecture, chapter 6 - Transcription in prokaryotes
Today we started the chapter on transcription.
We discussed the basics of transcription in prokaryotes, including some terminology specific to the transcription process and features necessary for it (e.g. roles of promoter and terminator regions)
Reminders:
- Labs 2 and 3 are due today
- We have our first exam on Friday
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