Today we started the phenol-chloroform isoamyl alcohol (PCI) DNA extraction from most students' blood. Blood samples were obtained throughout Monday and Tuesday.
Students added SSC buffer (pH stabilization), SDS (cell lysis), sodium acetate (NaOAc; protein precipitation), proteinase K (inactivation of endonucleases), PCI (separation of proteins and nucleic acids), and 100% ethanol (DNA precipitation). Samples were frozen to continue the process tomorrow.
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