Today we had a discussion in which we summarized some of the most important techniques that are used to study proteins and nucleic acids. Topics that were covered:
- Protein purification
- Denaturing PolyAcrylamide Gel Electrophoresis (SDS-PAGE)
- Two-dimensional electrophoresis
- PCR
- PCR in disease diagnosis
- DNA sequencing
- Chain termination method (Sanger method - manual and automated)
- Shotgun sequencing
- Pyrosequencing
- Next-generation sequencing
- DNA typing
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